Biological Analysis

At Ascentia, we provide a wide range of biological assays and disease-relevant models to support drug discovery programs focused on small molecule compounds. Our services include:

• Evaluation of small molecule activity
  using genetically modified cell lines and enzymatic assays
• Permeability studies
  assessing the ability of small molecules to cross biological membranes
• Cytotoxicity profiling
  to determine compound-induced cell death across various cell types
• Assessment of cell proliferation and migration
  particularly in cancer cell models, to study compound effects on tumor progression
• Secretome analysis
  investigating how small molecules influence the secretion profile of both normal and cancer cells
• Mechanism of action studies
  using advanced techniques such as*:
  ▪️ Real-time PCR
  ▪️ ELISA
  ▪️ Flow cytometry
  ▪️ Western blotting
  ▪️ Confocal microscopy
  ▪️ And other molecular biology methods
• In vivo studies
  in murine models of cancer and inflammatory diseases, enabling translational insights and preclinical validation
• Biophysical measurements of small molecule – macromolecular target affinity*
  ▪️ SPR
  ▪️ DSF

*services provided in cooperation with Business Partners